Romesberg's semi-synthetic organism (2014)
Floyd Romesberg's lab at Scripps Research created a semi-synthetic organism with an unnatural base pair (d5SICS-dNaM) in its DNA. The bacterium E. coli replicated the synthetic pair alongside its natural DNA.
The semi-synthetic organism showed that cells can replicate DNA with unnatural letters.
It demonstrated the feasibility of expanded genetic systems in living cells, though the pair was later refined.
Romesberg's work showed replication of unnatural letters in cells; the new study addresses transcription, the next step in gene expression.
